Screening concepts for the isolation of biosurfactant. Biosurfactant from microorganisms was proven to be an alternative source over chemical surfactant due to its structural and functional similarity and biodegradability properties. Two bacterial strains were isolated having ability to produce biosurfactant. Isolation and characterization of biosurfactant producing. Characterization of bacteria isolation of bacteria from. The combination of oildegrading bacteria and biosurfactant or biosurfactantproducing bacteria has also been tested by research groups. Screening, isolation and characterization of biosurfactant producing. The isolates showed an exponential growth in the medium. Only five strains, all of them haemolytic, tested positive. Biosurfactant production was assessed by such parameters as drop collapse, haemolysis, emulsification and oil spreading test. Occurrence of biosurfactantproducing bacteria in hydrocarbonpolluted environments was reported by many researchers yateem et al. Bacteria are the main group of biosurfactant producing microorganisms, although they are also produced by some yeasts and filamentous fungi. It was presumed that if the cell free culture broth contains biosurfactant then it would emulsify the hydrocarbons present. Isolation of biosurfactant producing bacteria from.
Characterization and phylogenetic analysis of biosurfactant. Research projects seeking funding isolation and characterization of biosurfactant producing bacteria from oil polluted environment. Isolation and selection of biosurfactantproducing bacteria. Ten out of thirty strains showed the biosurfactant activity. The goal of this study was isolation and characterization of biosurfactant producing bacteria from caspian sea water. The highest biosurfactant production excreted and cellbound biosurfactants was achieved with lactobacillus paracasei ssp.
Ha, mdc, tgst efficiently detected 15, 17 and 14 biosurfactant producers respectively. Isolation of biosurfactant producing bacteria from garbage. The use of selective media loaded with carbon sources sucrose 10 g. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. Emulsification assay is an indirect method used to screen biosurfactant production. Characterization, antimicrobial and metal remediation properties. Here, crude oil was used as the hydrophobic substrate. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran 2 biotechnology center of iranian research organization for science and technology, iran 3research institute of petroleum industry, tehran, iran. Ten morphologically distinct microbial colonies were isolated and. A 5g sample of each soil was placed into a 250ml flask containing 50 ml of tap water and incubated at 23c on a shaker at 200 rpm for 21 days. In well diffusion assay, biosurfactant produced a zone of inhibition against s. After screening, candidate biosurfactant producing bacteria were selected for optimization of the physical and.
The values are meaning of three readings, emulsification index 30% is indicated in bold to show high activity. Abstract biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. The present study aimed the isolation of a potent biosurfactant producing indigenous bacteria which can be employed for crude oil remediation. Qualitative analysis of biosurfactant production was identified by performing assays like hemolysis on blood agar, penetration assay and ctab. Physical characterization of bacterial isolates 11. Bacteria are the main group of biosurfactantproducing microorganisms, although it is also produced by some yeast and filamentous fungi. Isolated microorganisms were identified based on the biochemical characterization as. Affandi ie, suratman nh, abdullah s, ahmad wa, zakaria za 2014 degradation of oil and grease from highstrength industrial effluents using locally isolated aerobic biosurfactantproducing bacteria. Screening and identification of biosurfactant bioemulsifier producing bacteria from crude oil contaminated sites of gujarat, india s. Only 37 of 492 strains of different origins had haemolytic activity. A number of microorganisms have been stated to produce a number of classes of.
Sixty nine hydrocarbon utilizing bacteria isolated from selected crude oil contaminated sites of ongc gujarat were screened for biosurfactant producing potential. Assessment of different screening methods for selecting. From four different sites of lonar lake 12 sediment, matt and water samples were collected and screened on mineral salt medium containing 2% soybean oil as a carbon source. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, halomonas sp bs4 was confirmed by 16 s rrna sequencing. The biosurfactant showed significant emulsifying index, drop. Distribution of biosurfactant producing bacteria was abundant in various season water and sediment. In recent time, biosurfactants produced by diverse group of microorganisms are. Pdf isolation of biosurfactant producing bacteria from oil. During the production of biosurfactants their growth in the culture medium was assessed by optical density at the wavelength of 600nm. In the present study, a biosurfactant producing candida parapsilosis strain was isolated and identified by our laboratory. Isolation, characterization and antibacterial effect of.
Accordingly, the aims of this study were to isolate and characterize of novel biosurfactant producing bacteria isolated from palm oil contaminated soils in palm oil industry. Guanabara bay and its mangrove protection areas are constantly subject to a multiple source of petroleum. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. Pdf isolation of biosurfactant producing bacteria from. Nov 11, 2016 affandi ie, suratman nh, abdullah s, ahmad wa, zakaria za 2014 degradation of oil and grease from highstrength industrial effluents using locally isolated aerobic biosurfactant producing bacteria.
Biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. Biosurfactant producing bacterial density was observed for a period of one year december 2009 to november 2010. To confirm the ability of isolates in biosurfactant production, haemolysis test, emulsification test and measurement of surface tension were conducted. Culturable screening for biosurfactantproducing isolates. The potential application of these bacteria in petroleum industry was investigated. Isolation of biosurfactant producing bacteria from petroleum. Most biosurfactant producing microorganisms reported are from the genus pseudomonas, candida and bacillus is known to produce rhamnolipids, glycolipids and lipopeptide surfactants 3, 11. Biosurfactantproducing bacteria were found to constitute a significant.
Isolation of biosurfactant producing bacteria from oil. Nov 01, 2002 culturable screening for biosurfactantproducing isolates. Biosurfactants contains hydrophobic and hydrophilic moieties that reduce the surface tension and interfacial tension by accumulating at the interface of hydrophobic substances and thus increase the solubility, bioavailability and subsequent biodegradation of the hydrophobic or insoluble organic compounds. Isolation of biosurfactant producing exiguobacterium sp. Research article isolation, screening and characterization of. They were present in water and sediment samples respectively in the density of 10 2 and 10 3 cfumlg. Antibiotic sensitivity test for biosurfactant producing bacteria 8. Considering leakage of crude oil from tankers in harbors and other anthropogenic activities, we suspected the occurrence of biosurfactantproducing bacteria at our sampling site ebrahimi et al. Screening, isolation and characterization of biosurfactant. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactantproducing bacteria. Bacterial quantification bq quantification was performed following carlucci et al. The word surfactant is derived from the term surface active agent.
Screening and optimization of biosurfactant production by. Isolation and characterization of biosurfactantproducing. Screening and characterization of biosurfactantproducing. Identification of biosurfactant producing bacteria. In light of this, the work embarked with an attempt to isolate potential biosurfactant producing bacteria from various processes involved in the milling of. In addition, lowcost substrates for biosurfactant production were evaluated.
The growth optimization of halomonas sp bs4 revealed their optimum growth at 8% nacl and 68 ph in the growth medium. In light of this, the work embarked with an attempt to isolate potential biosurfactantproducing bacteria from various processes involved in the milling of. The search for biosurfactantproducing microorganisms in the pinyon plant jatropha curcas l. After screening, candidate biosurfactantproducing bacteria were selected for optimization of the physical and. Biosurfactant producing bacteria, biosurfactant, emulsification, microbial enhanced oil.
The biosurfactant producing ability of bacteria was investigated by oil displacement agar plate and emulsification index method. These 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. According to these physiological roles, biosurfactant producing microbes can be found in different environments. Isolation and characterization of biosurfactant production under. Distribution of biosurfactantproducing bacteria in. L1 showed it was useful in terms of screening and maintenance of biosurfactantproducing bacteria from a highly eutrophic environment. Present study focused on biosurfectant producing bacteria from lonar lake.
Most biosurfactantproducing microorganisms reported are from the genus pseudomonas, candida and bacillus is known to produce rhamnolipids, glycolipids and lipopeptide surfactants 3, 11. This strain is potential candidate for microbial enhanced oil recovery. The presence of biosurfactant was determined by the dropcollapse test, microplate analysis, oil spreading technique, emulsification index %ei 24 and surface tension. Among 64 bacterial strains isolated in this study, the best two of biosurfactant producing bacteria were selected and identified based on the phenotypic properties and molecular approach based on. Crude oil is a highly hydrophobic material with most of its components having low water solubility kuyukina et al. Alternative methodology for isolation of biosurfactant. These compounds can be synthesized by microorganisms growing on waterimmiscible hydrocarbons, as well as on watersoluble compounds such as glucose, sucrose, glycerol, or ethanol, and can be excreted or. Screening and identification of biosurfactant bioemulsifier. Isolation of biosurfactant producing bacteria braz. The direct isolation of the microorganisms was carried out using serial dilution up to 10. The oil contaminated soil samples were collected from varachha, surat. Among 64 bacterial strains isolated in this study, the best two of biosurfactantproducing bacteria were selected and identified based on the phenotypic properties and molecular approach based on. The strain4 biosurfactant component was mainly glycolipid in nature.
A number of studies have reported the potential of bacillus species as biosurfactant producers and they produce lipopeptide type of biosurfactant nitschke and pastore, 2004. The e24 of all three organisms are noted in table 3. Original article characterization and phylogenetic analysis of biosurfactantproducing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand. Biosurfactant production was screened in four lactobacilli strains. Biosurfactants are produced by a variety of microorganisms mainly bacteria, fungi and yeasts are diverse in chemical composition and their nature and the amount depend on the type of microbes producing a particular biosurfactant. Higher concentrations of biosurfactant led to increase in the diameter of zone of inhibition. Bacteria are the main group of biosurfactant producing microorganisms, although it is also produced by some yeast and filamentous fungi. Many biosurfactant producing microbes were isolated from soils or water samples which are contaminated with hydrophobic organic compounds like e. Total 30 bacterial strains were isolated from samples of soil contaminated with petroleum and its products.
Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant producing bacteria. These molecules reduce surface tension both aqueous solutions and hydrocarbon mixtures. In this study biosurfactant producing bacteria were isolated from 50 terrestrial samples collected in areas contaminated with petroleum in several districts in bangkok, thailand. Research article isolation, screening and characterization. Physiological and molecular characterization of biosurfactant. Screening and identification of biosurfactant bioemulsifier producing bacteria from crude oil contaminated sites of gujarat, india. Ruwaida kuwait institute of scientific research, p.
Biosurfactants or surfaceactive compounds are produced by microoaganisms. When oil spread in the environment, most of the oil hydrocarbons remain. The biosurfactant producing bacteria were isolated from oil contaminated site. The annual release of crude oil in the oceans is estimated to be around 1. View the article pdf and any associated supplements and figures for a period of 48 hours. And in resulting wide variety of biosurfactant can be produced accordingly to the demand and uses. Only 37 of 492 strains of different these 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. Different biosurfactant screening tests such as drop collapse, oil spreading, emulsification index and hemolytic activity confirmed the production of biosurfactant by the isolated candida parapsilosis strain. Isolation and screening of biosurfactant producing bacteria from oil contaminated soils in iraq doi. Biosurfactant plays an important role in bioremediation of crude oil contamination and microbial enhanced oil recovery meor. Occurrence of biosurfactant producing bacteria in hydrocarbonpolluted environments was reported by many researchers yateem et al. Isolation and enrichment of biosurfactant producing bacteria two biosurfactant producing bacterial strains were isolated and enriched from diesel oil contaminated soil in dindigul 10. Isolation and screening of biosurfactant producing bacteria. Vijaya,et at 20isolated 42 isolates and selected for biosurfactant production.
One biosurfactantproducing bacteria achromobacter sp. Isolation of biosurfactant producing bacteria from garbage soil. Ten selected biosurfactant producing strains were screened for biosurfactant production by numerous tests. Kleber encyclopedia of life support systems eolss biosurfactant producing organism csource localization1 charge surface tension mn m1 cmc mg l1 interfacial tension mn m1 cellobioselipids ustilago sp.
Ghayyomi et al 2012 isolated a strain of bacillus sp. Apr 10, 20 halophilic bacteria were isolated from thamaraikulam solar salt works in india. Isolation and screening of biosurfactant producing. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. All of the bacterial isolates were grown in mineral salt medium msm with addition of 1% vv tapis crude oil as carbon source. Fortyfive strains of bacteria, comprising 18 acinetobacter and 27 other bacteria along with positive mtcc reference strains were examined. The combination of oildegrading bacteria and biosurfactant or biosurfactant producing bacteria has also been tested by research groups.
1098 151 224 321 80 1352 402 512 1315 531 409 1617 1589 1292 1191 1357 1027 1317 853 509 31 1008 1515 715 1467 957 628 817 1438 97 936 239 363 902 1397